A micro-fluidic device having a cavity containing a solid support (e.g. , a plurality of particles) and a biomolecule immobilized on a surface of the solid support is provided. The micro-fluidic device can be used to monitor interactions between a biomolecule in a sample and the biomolecule immobilized on the solid support. For example, the micro-fluidic device can be used to monitor protein self-interactions wherein the protein in the sample is the same protein as that immobilized on the surface of the solid support. A method of making the micro-fluidic device is also described. The micro-fluidic device can be used for chromatographic applications where sample consumption is crucial.
Wilson, Wilbur W., Charles S. Henry, Carlos D. Garcia