Disclosed herein are a high-throughput continuous culture system and novel methodologies for the experimental evolution of natural and synthetic microbes using the continuous culture system. The microbial culture is exposed to a stress ramp function which is overlaid on top of a culture fitness function. The amount of stress applied to the culture is increased in response to increased fitness of the microbial culture.
Bashor, Caleb J., Y. A. N. G. Jason Hung-ying, Arnaud GUTIERREZ, A. H. N. Wooseok Steven, James J. Collins, W. O. N. G. Branton Gei-Chin, Ahmad S. Khalil