A G Protein Coupled Receptor (GPCR) is incorporated in polymeric giant unilamellar protein vesicles (pGUPs). By utilizing an agarose rehydration technique, the GPCR is inserted in the biased, physiological orientation with the C-terminus cytosolic and N-terminus extracellular. The GPCR is fully functional within the polymeric bilayer, exhibiting physiological responses to various ligands. The entire population of GPCRs in pGUPs remains fully functional after lyophilization for 120 hours.
Gutierrez, Mary G., Noah Malmstadt, Carson Riche