Hemagglutination is utilized for various immunological assays, including blood typing and virus detection. Herein, we describe a method of rapid hemagglutination detection based on a microfluidic channel installed on an optical waveguide-mode sensor. Human blood samples mixed with hemagglutinating antibodies associated with different blood groups were injected into the microfluidic channel, and reflectance spectra of the samples were measured after stopping the flow. The agglutinated and nonagglutinated samples were distinguishable by the alterations in the irreflectance spectra with time; the microfluidic channels worked as spatial restraints for agglutinated red blood cells. The demonstrated system allowed rapid hemagglutination detection within 1min. The suitable height of the channels was also discussed.
Ashiba, Hiroki, Makoto Fujimaki, Koichi Awazu, Mengying Fu, Yoshimichi Ohki, Torahiko Tanaka, Makoto Makishima
Jpn. J. Appl. Phys.