Cell migration on micropatterned substrates is a complex process regulated by cell-substrate interactions. This paper presents a passive method for guiding the migration of NIH 3T3 fibroblasts using consecutive trapezoidal micropatterns, made of a biocompatible photopolymer (OrmoComp), to provide tapered micro-features on the cells. The cell migration paths measured from our platforms quantitatively address the dependence of the migratory behavior of the cells on the tapered micro-features. Most of the cells on the micropatterns have moved in a converging direction rather than in a diverging one; migration speed on the micropatterns has been noticeably faster than that on flat substrates.
Han, Eui Don, Sang-Hee Yoon, Byeong Hee Kim, Young Ho Seo
Appl. Phys. Lett.