HARRICK PLASMA

A micropatterned microfluidic device for engineering soluble and tethered environmental cues in cell culture

We report a facile technique to integrate micropatterns of any shape within microfluidic devices. The technique involves protecting the region of a glass surface printed with micropatterns during plasma treatment, allowing the unprotected regions to be bonded to PDMS. In this manner, the micropatterned region is incorporated within a PDMS-based microfluidic device. Using a gradient-generating microfluidic device, we show that micropatterns of complex shapes can be aligned to spatial gradients in a soluble factor.

Natividad, R. J., Anand R. Asthagiri

2014 40th Annual Northeast Bioengineering Conference (NEBEC)

1--2

2014

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