HARRICK PLASMA

A microfluidic device for epigenomic profiling using 100 cells

The sensitivity of chromatin immunoprecipitation (ChIP) assays poses a major obstacle for epigenomic studies of low-abundance cells. Here we present a microfluidics-based ChIP-seq protocol using as few as 100 cells via drastically improved collection of high-quality ChIP-enriched DNA. Using this technology, we uncovered many new enhancers and super enhancers in hematopoietic stem and progenitor cells from mouse fetal liver, suggesting that enhancer activity is highly dynamic during early hematopoiesis.

Cao, Zhenning, Changya Chen, Bing He, Kai Tan, Chang Lu

Nature Methods

12

959-962

2015

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