Microfluidic devices enable the modeling of large systems or system components on a microscopic scale, increasing the potential for automation and portability while reducing experimental measurement times and costs. This article discusses the benefits of plasma treatment for microfluidic device fabrication, its applications, and some processing guidelines when using our plasma instruments.
For references citing the use of our plasma cleaners in microfluidic applications, see the Microfluidic Devices category in the References: Technical Articles page.
Poly(dimethylsiloxane) (PDMS) is most commonly used material for building microfluidic devices. Plasma treatment quickly renders PDMS surfaces hydrophilic through plasma oxidation [Figure 1]. Following plasma treatment, the PDMS-PDMS or PDMS-glass surfaces may be bonded and sealed irreversibly to create leak-tight channels. Microfluidic channel surfaces may also be rendered hydrophilic to enhance fluid flow and wetting of channels. In addition, alternating hydrophilic-hydrophobic regions may be patterned on microfluidic surfaces by plasma treating devices through a patterned mask.
Microfluidic surfaces and channels can be plasma treated without affecting the bulk properties of the device. As such, plasma treatment has been used to facilitate the fabrication of microfluidic devices for applications such as:
After patterning a PDMS substrate by replica molding from a master mold, the PDMS is oxidized in air oxygen (O2) plasma. An air or O2 plasma removes organic, hydrocarbon material by chemical reaction with highly reactive oxygen radicals and ablation by energetic oxygen ions. This leaves silanol (SiOH) groups on the surface, rendering the surface more hydrophilic and increasing surface wettability. Following plasma activation, the PDMS is immediately placed in contact with another oxidized PDMS or glass surface to form bridging Si-O-Si bond at the interface, creating an irreversible seal.
Below are suggested process conditions for plasma activation of PDMS-PDMS or PDMS-glass in a Harrick Plasma cleaner (some experimentation may be required to determine optimal process conditions):
 Norred SE, Caveney PM, Retterer ST, Boreyko JB, Fowlkes JD, Collier CP and Simpson ML. "Sealable femtoliter chamber arrays for cell-free biology.” J. Vis. Exp. (2015) 97: e52616.
 Bhattacharya S, Datta A, Berg JM, Gangopadhyay S. "Studies on surface wettability of poly(dimethyl) siloxane (PDMS) and glass under oxygen-plasma treatment and correlation with bond strength." J. Microelectrom. S. (2005) 14(3): 590-597.
 Hillborg H, Ankner JF, Gedde UW, Smith GD, Yasuda HK, Wikström K. "Crosslinked polydimethylsiloxane exposed to oxygen plasma studied by neutron reflectometry and other surface specific techniques." Polymer (2000) 41: 6851-6863.
 Duffy DC, McDonald JC, Schueller OJA and Whitesides GM. "Rapid prototyping of microfluidic systems in poly(dimethylsiloxane)". Anal. Chem. (1998) 70: 4974-4984.
Data from Jiang, X., H. Zheng, S. Gourdin, P. T. Hammond. "Polymer-on-Polymer Stamping: Universal Approaches to Chemically Patterned Surfaces." Langmuir (2002) 18: 2607-2615; Zheng, H., M. F. Rubner, P. T. Hammond. "Particle Assembly on Patterned "Plus/Minus" Polyelectrolyte Surfaces Via Polymer-On-Polymer Stamping." Langmuir (2002) 18: 4505-4510.